Reporter

Part:BBa_K1470004:Design

Designed by: Dennis Zimmer   Group: iGEM14_Freiburg   (2014-10-08)

Secreted Alkaline Phosphatase (SEAP)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 209
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 520
    Illegal NgoMIV site found at 1489
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 850
    Illegal BsaI.rc site found at 1339


Design Notes

Design


Source

AG Weber Institute of Biology II / BIOSS Center for Biological Signalling Studies Albert-Ludwigs-Universität Freiburg Germany

References

[X] Maxam AM, Gilbert W (1980). „Sequencing end-labeled DNA with base-specific chemical cleavages“. Meth. Enzymol. Methods in Enzymology 65 (1): 499–560. [Y] Lowe ME. „Site-specific mutations in the COOH-terminus of placental alkaline phosphatase: a single amino acid change converts a phosphatidylinositol-glycan-anchored protein to a secreted protein“. J Cell Biol. 1992 Feb;116(3):799-807.